Benefits[edit | edit source]
Methods[edit | edit source]
Method #1[edit | edit source]
Firstly, start by splitting the mushroom stem in half longitudinally. It needs to be sterile on the inside, so don't cut it in half, dragging contaminants from the outside to the inside. Once you have split the fruit, sterilize your scalpel and let it cool down really well (this is especially important when taking tiny tissue samples which could be fried by the heat).
- The black is the split mushroom stem. The grey in the middle is the hollow section of the stem.
- The red lines are where you make your first cuts. Make sure to avoid the outer non-sterile parts. Also make sure you don't make such a deep cut that your scalpel pierces through the stem, contaminating everything. It's a delicate art but it's not too difficult, especially once you get the hang of it.
- The green line is the final cut. After this the piece of tissue will be almost free.
- The blue line is where you place the tip of the scalpel blade to remove the piece. The diagram features a Swann Morton, type 20 blade, and the direction in which to insert and pull. This can take a bit of practice.
When pulling the tissue out, there will be tissue hairs connecting it to the stem that need to detach, but if you've done everything right, you should be able to pull it away without major problems. Then just place it on agar and hope for the best!
- The longer the tissue sample you take, the higher the chance you'll be able to isolate clean mycelium, as it's unlikely that if contamination happens, it's going to occur around the entire length of the tissue strip.
- Practice makes perfect. Don't worry if the tissue slips off the scalpel as you're trying to remove it, it happens. If you want, you can start over and make a new cut in a different spot, or just try your luck.
- When cloning mushrooms from the wild, be careful not to choose tissue from worm-infested areas of the stem. These will definitely harbor contamination. Split all the way up to the cap if necessary.
Credit to Shroomery user Adas